degenerate oligonucleotide

基本解释

Objective To investigate the possibility of applying degenerate oligonucleotide primer PCR(DOP-PCR) and comparative genomic hybridization(CGH) in analysing genomic genetics of a single cell.
目的探索单细胞退变寡核甘酸引物PCR（DOP-PCR） -比较基因组杂交（CGH）技术应用于单细胞全基因组分析及着床前胚胎遗传学研究的可能性。
A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR.
根据肺炎支原体细胞粘附素蛋白质的基因保守区两侧序列，设计并合成一对变性寡核苷酸引物L2R2，用于以鸡毒支原体S6株基因组DNA为模板的PCR反应。