gradient elution

基本解释

A novel method for the fast optimization of binary multi-step gradient elution in reversed phase-high performance liquid chromatography was proposed.
提出了一种反相高效液相色谱中二元多台阶梯度分离条件快速优化方法。
Objective:To study on chromatography fingerprint(CFP) of Huangqi herbs. Method: HPLC was used on a Hypersil ODS column with water-acetonitriles gradient elution.
应用高效液相色谱法研究了黄芪的指纹图谱，并对毛蕊异黄酮、芒柄花素及黄芪甲苷进行了含量测定。
Method The main ingredient of the injection, Red Ginseng, was determined by HPLC and used binary solvent-delivery gradient elution to control the main component in Red Ginseng.
方法采用HPLC法，以君药红参中人参皂苷为检测指标，采用梯度洗脱的方法，控制其中红参主要成分的色谱峰。

梯度洗脱
It was proved that the model could describe the linear gradient elution of protein under non-linear adsorption condition fairly well.
该模型可以较好地预测非线性吸附条件下蛋白质的线性梯度洗脱过程。
梯度淋洗
With hexane-isopropanol-acetic acid as mobile phase and by gradient elution method,the o-, m-, p- dihydroxybenzene isomers, phenol and p-benzoquinone can be separated fairlywell- The recovery of catechol is higher than 98%, the relative standard deviation is lessthan 0.
采用硅胶柱：以正己烷-异丙醇-醋酸力流动相，梯度淋洗，实现了邻苯二酚、对苯二酚、间苯二酚异构体、苯酚与对本醌的良好分离。
梯度冲洗
梯度淘析
梯度冲提

梯度洗脱
Methods:These dyes were extracted from the samples with n-Hexane and analyzed by gradient elution. The chromatographic separation was carried out on a reverse phase C18 column.
方法:样品中的对位红和苏丹红经正己烷提取、采用光谱分析选择合适测定波长、梯度洗脱、C18柱分离,外标法定量。
医药科学
等度洗脱